About measurement, accuracy, and calibration
Many Dino-Lite microscope and Dino-Eye camera models include 2-dimensional measurement features. These features are available on most USB models. A full compatibility chart can be found below. Analog (TV) models do not include measurement features. The measurement features are used by selecting the appropriate tool from within the software and then clicking on points on the image. Keyboard arrows can also be used to more precisely adjust lines by one pixel at a time. Measurement tools include line, continous line, fixed-length line, edge-detection line, point-to-line, polygon, circle, arc, angle, grid and several others.
The magnification values provided on the Dino-Lite device itself are given as reference. For accurate measurement results, a calibration target is included with all models that support measurement features. The calibration feature in the software should be used together with the calibration target prior to performing measurements. The Dino-Lite is an affordable tool designed to offer a broad range of features for multiple applications. However, when used calibrated properly accuracy results can often match precision measuring equipment.
Accuracy depends on several factors including magnification, screen resolution, and proper calibration. Since Dino-Lite microscopes provide variable magnification, the tolerance will be different depending on what magnification you are using. For example, If you are using a standard magnification model at 220x with a screen resolution of 1920x1080, you can expect an accuracy of 0.001mm and If you are using a high magnification model at 930x with the same screen resolution, you can expect an accuracy of 0.3um. For more accuracy examples, please see the images below.
50x / 1.3MPHorizontal FOV [accuracy]: 9.564mm [+/- 0.192mm]
One pixel increment (one keyboard arrow press): ~7.4um
200x / 1.3MPHorizontal FOV [accuracy]: 2mm (2000um) [+/- 0.04mm (40um)]
One pixel increment (one keyboard arrow press): ~1.5um
950x / 1.3MPHorizontal FOV [accuracy]: 400um [+/- 8um]
One pixel increment (one keyboard arrow press): ~0.3um
To calibrate, use the included calibration target or another item of known dimensions. Select the "Calibration" button on the right side of the toolbar and follow the on-screen instructions. For more help see the link below titled "How to calibrate".
Currently we are unaware of any institutions that will certify variable magnification digital microscope's calibration for measurements. However, it is possible to calibrate measurement capable Dino-Lite models with a certified calibration target from a third party.
Instead of using the Dino-Lite provided courtesy reference calibration target, some users may purchase a certified calibration from a third party party. Here are some third-party external sources that we are aware of:https://www.maxlevy.com/category/microrules.html