How to use Dino-Lite’s DPQ feature to gather depth information

DPQ Depth Information Guide with compatible Edge models

DinoCapture 3.0 or 2.0 and selected Dino-Lite models offer an improved and more accurate functionality for the acquisition of depth information. Now, users can acquire depth information from the focus shift between two focal planes during live imaging.

This functionality only applies to the following compatible models that have EDOF:

SeriesModel
EdgePLUSAM4917MZT, AM4917MZTL, AM4917MZT4, AM4917MT8, AM8917MZT, AM8917MZTL
Edge 5MPAF7915MZT, AF7915MZTL
Edge 3.0AM73915MZT (R10), AM73915MZTL (R10A), AM73915MZT4, AM73915MT8

DinoCapture 3.0 or 2.0 ver. 1.5.40 or newer version required. Click here to download the latest version. Contact us for more information.

StepReference
1 Adjust the focus to a plane of interest.
This position will be considered the reference plane as 0.
1a
To acquire depth information, readjust the focus by:

Middle-click (mousewheel click) on a point of interest to autofocus, if necessary, scroll the mouse wheel to re-adjust the focus.

or

Press the “Ctrl” key and scroll the mouse wheel to change focus manually. The depth information is shown as the focus shift.
2a or “Ctrl” + 2b
2c
This cursor 2d indicates that refocus mode is active and that the focus can be readjusted. A positive focus shift refers to a plane below the reference plane.
To exit Refocus mode
Left-click anywhere on the preview window or press the “ESC” key.
3a3b
Still have questions? We’re here to help.Contact our technical support team

Depth Acquisition: Maximum Depth Range and Accuracy

When performing depth acquisitions, its maximum depth range and tolerance are determined by the magnification being used. This section shows the variations in maximum depths and tolerance at different magnifications in AM4917 models.

Maximum Depth Range

The maximum measurable depths that the device can perform is referred to as maximum depth range. Usually, the higher the magnification, the lower the maximum depth range, and vice versa.

depth range

Tolerance

The tolerance is the allowable variance in depth acquisitions. The higher the magnification, the lower the tolerance, and vice versa.

tolerance
AM4917MZT / AM73915MZT Focus Shift
focus shift zt4
AM4917MZT4 / AM73915MZT4 Focus Shift
focus shift mt8
AM4917MT8 / AM73915MT8 Focus Shift

Table with focus shift parameters of AM4917MZT and AM4917MZTL

Below is a table providing all the relevant parameters when acquiring depths with AM4917MZT and AM4917MZTL microscope models.

AM4917MZT and AM73915MZT(R10)
MagnificationMax. Depth Range (+/- mm) (Down)Max. Depth Range (+/- mm) (Up)Tolerance (+/- mm)
2028.1419.000.146
3013.0610.100.083
407.996.580.058
505.604.790.045
604.253.730.038
703.413.040.033
802.812.550.029
902.392.190.027
1002.071.910.025
1101.821.690.023
1201.621.510.022
1301.461.370.020
1401.321.240.02
1501.191.130.019
1601.091.040.019
1701.000.950.018
1800.920.880.017
1900.850.820.017
2000.780.750.017
2100.720.700.016
2200.670.650.016
AM4917MZTL and AM73915MZTL(R10A)
MagnificationMax. Depth Range (+/- mm) (Down)Max. Depth Range (+/- mm) (Up)Tolerance (+/- mm)
2034.9425.250.251
3016.8913.620.149
4010.659.040.108
507.596.650.087
605.835.220.074
704.694.260.065
803.913.590.059
903.343.090.054
1002.902.700.051
1102.552.400.048
1202.282.160.046
1302.051.940.044
1401.841.750.042
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